Steven Clarke Lab Publications – 2012 – Present
316. Pang, E. Z., Zhao, B., Flowers, C., Oroudjeva, E., Winter, J. B., Pandey, V., Sawaya, M. R., Wohlschlegel, J., Loo, J. A., Rodriguez, J. A., and Clarke, S. G. (2025) “Structural basis for L-isoaspartyl-containing protein recognition by the human PCMTD1 cullin-RING E3 ubiquitin ligase” J. Biol. Chem. 301, article 110735, pp.1-17. (DOI: 10.1016/j.jbc.2025.110735). PMID: 40975169. PMCID: PMC12590147. [bioRxiv (Preprint) 2025 May 21:2025.05.21.654933. doi: 10.1101/2025.05.21.654933. PMID: 40475564. PMCID: PMC12139721.]
315. Lowe, T. L., Valencia, D. A., Velasquez, V. E., Quinlan, M. E., and Clarke, S. G. (2024) “Methylation and phosphorylation of formin homology domain proteins (Fhod1 and Fhod3) by protein arginine methyltransferase 7 (PRMT7) and Rho Kinase (ROCK1)” J. Biol. Chem. 300, article 107857, pp. 1-15. (DOI: 10.1016/j.jbc.2024.107857). PMID: 39368550. PMCID: PMC11584945.
314. Clarke, C. and Clarke, S. (2024) “Retrospective – Daniel E. Atkinson (1921-2024)” ASBMB Today Magazine. Online publication available at: https://www.asbmb.org/asbmb-today/people/052724/daniel-e-atkinson-1921-2024
313. Clarke, S. G. and Eisenberg, D. S. (2024) “Paul Delos Boyer: A Biographical Memoir” Biographical Memoirs, National Academy of Sciences. Online publication available at: https://www.nasonline.org/publications/biographical-memoirs/memoir-pdfs/boyer-paul.pdf.
312. Holtz, A. G., Lowe, T. L., Aoki, Y., Kubota, Y., Hoffman, R. M., and Clarke, S. G. (2023) “Asymmetric and Symmetric Protein Arginine Methylation in Methionine-Addicted Human Cancer Cells” PLoS ONE 18, e0296291. DOI: 10.1371/journal.pone.0296291.
311. Yang, M.-L., Lam, T. T., Kanyo, J., Kang, I., Zhou, S. Z., Clarke, S. G. and Mamula, M. J. (2023) “Natural isoaspartyl modification of ZAP70 alters T cell responses in lupus” Autoimmunity 1, 2282945. DOI: 10.1080/08916934.2023.2282945.
310. Feoli, A., Iannelli, G., Cipriano, A., Milite, C., Shen, L., Wang, Z., Hadjikryiacou, A., Lowe, T. L., Safaeipour, C., Viviano, M., Sarno, G., Morretta, E., Monti, M., Yang, Y., Clarke, S. G., Cosconati, S., Castellano, S., and Sbardella, G. (2023) “Identification of a Protein Arginine Methyltransferase 7 (PRMT7)/Protein Arginine Methyltransferase 9 (PRMT9) Inhibitor” J. Med. Chem. 66, 13665-13683. DOI: 10.1021/acs.jmedchem.3c01030. Selected for cover illlustrative art.
309. Bondoc, T. J., Lowe, T. L., Clarke, S. G. (2023) “The exquisite specificity of human protein arginine methyltransferase 7 (PRMT7) toward Arg-X-Arg sites” PLoS ONE 18, e0285812. DOI: 10.1371/journal.pone.0285812.
308. Yang, M. L., Connolly, S. E., Gee, R. J., Lam, T. T., Kanyo, J., Peng, J., Guyer, P., Syed, F., Tse, H. M., Clarke, S. G., Clarke, C. F., James, E. A., Speake, C., Evans-Molina, C., Arvan, P., Herold, K. C., Wen, L., Mamula, M. J. (2022) “Carbonyl Posttranslational Modification Associated With Early-Onset Type 1 Diabetes Autoimmunity” Diabetes 71, 1979-1993. DOI: 10.2337/db21-0989.
307. Lowe, T. L. and Clarke, S. G. (2022) “Human protein arginine methyltransferases (PRMTs) can be optimally active under nonphysiological conditions” J. Biol. Chem. 298, 102290. DOI: 10.1016/j.jbc.2022.102290.
306. Warmack, R. A., Pang, E. Z., Peluso, E., Lowenson, J. D., Ong, J. Y., Torres, J. Z., Clarke, S. G. (2022) “Human Protein-L-isoaspartate O-Methyltransferase Domain-Containing Protein 1 (PCMTD1) Associates with Cullin-RING Ligase Proteins” Biochemistry 61, 879-894. DOI: 10.1021/acs.biochem.2c00130.
305. Yamamoto, J., Inubushi, S., Han, Q., Tashiro, Y., Sugisawa, N., Hamada, K., Aoki, Y., Miyake, K., Matsuyama, R., Bouvet. M., Clarke, S. G., Endo, I., Hoffman, R. M. (2022) “Linkage of methionine addiction, histone lysine hypermethylation, and malignancy” iScience 25, 104162. DOI: 10.1016/j.isci.2022.104162.
304. Clarke, S. G. “Guido Guidotti (1933 – 2021) Remembering a biochemist’s biochemist.” ASBMB Today, Mar. 2022, pp.9-12.
303. Yamamoto, J., Aoki, Y., Inubushi, S., Han, Q., Hamada, K., Tashiro, Y., Miyake, K., Matsuyama, R., Bouvet, M., Clarke, S. G., Endo, I., and Hoffman, R. M. (2022) “Extent and Instability of Trimethylation of Histone H3 Lysine Increases with Degree of Malignancy and Methionine Addiction” Cancer Genom. Proteom. 19, 12-18. DOI: 10.21873/cgp.20299.
302. Gladyshev, V. N., Kritchevsky, S. B., Clarke, S. G., Cuervo, A. M., Fiehn, O., de Magalhaes, J. P., Mau, T., Maes, M., Moritz, R., Niedernhofer, L. J., Van Schaftingen, E., Tranah, G. J., Walsh, K., Yura, Y., Zhang, B., and Cummings, S. R. (2021) “Molecular Damage in Aging” Nat. Aging 1, 1096-1106. DOI: 10.1038/s43587-021-00150-3.
301. Ayer, A., Fazakerley D. J., Suarna, C., Maghzal, G. J., Sheipouri, D., Lee, K. J., Bradley, M. C., Fernández-del-Rio, L., Tumanov, S., Kong, S. M. Y., van der Veen, J. N., Yang, A., Ho, J. W. K., Clarke, S. G., James, D. E., Dawes. I. W., Vance, D. E., Clarke, C. F., Jacobs, R. L., and Stocker, R. (2021) “Genetic screening reveals phospholipid metabolism as a key regulator of the biosynthesis of the redox-active lipid coenzyme Q” Redox Biol. 46, 102127. DOI: 10.1016/j.redox.2021.102127.
300. Soliman, R., Cordero-Maldonado, M. L., Martins, T. G., Moein, M., Conrotte, J.-F., Warmack, R.A., Skupin, A., Crawford, A. D., Clarke, S.G., and Linster, C.L. (2021) “L-Isoaspartyl methyltransferase deficiency in zebrafish leads to 1 impaired calcium signaling in the brain” Front. Genet. 11, 612343. DOI: 10.3389/fgene.2020.612343.
299. Dennis, T. N., Kang, A. S., Lowenson, J. D., Kenjic, N., Kirkwood, J. S., Clarke, S. G., and Perry J. (2020) “Human ARM1 structure and substrate specificity indicates that it is a DUF89 family damage-control phosphatase” J. Struct. Biol. 212, 107576. DOI: 10.1016/j.jsb.2020.107576.
298. White, J. T., Cato, T., Deramchi, N., Gabunilas, J., Roy, K. R., Wang, C., Chanfreau, G. F., and Clarke, S. G. (2019) “Protein methylation and translation: Role of lysine modification on the function of yeast elongation factor 1A” Biochemistry 58, 4997-5010. PMCID: PMC6913935.
297. Warmack, R. A., Shawa, H., Liu, K., Lopez, K., Loo, J., Horwitz, J., and Clarke, S. G. (2019) “The L-isoaspartate modification within protein fragments in the aging lens can promote protein aggregation” J. Biol. Chem. 294, 12210-12219. PMCID: PMC6690693.
296. Warmack, R. A., Boyer, D. R., Zee, C.-T., Richards, L. S., Sawaya, M. R., Cascio, D., Gonen, T., Eisenberg, D. S., and Clarke, S. G. (2019) “Structure of amyloid-β (20-34) with Alzheimer’s-associated isomerization at Asp23 reveals a distinct protofilament interface” Nature Commun. 10:3357. DOI: 10.1038/s41467-019-11183-z. PMCID: PMC6659688.
295. Jain, K., and Clarke, S. G. (2019) “PRMT7 as a unique member of the protein arginine methyltransferase family: A review” Arch. Biochem. Biophys. 665, 36-45. PMCID: PMC6461449.
294. Yang, M.-L., Doyle, H. A., Clarke, S. G., Herold, K. C., and Mamula, M. J. (2018) “Oxidative Modifications in Tissue Pathology and Autoimmune Disease” Antioxidants Redox Signaling 29, 1415-1431. PMCID: PMC6166690.
293. Clarke, S. G. (2018) “Retrospective: Paul D. Boyer (1918-2018)” ASBMB Today, August 2018 issue, pages 8-10.
292. Clarke, S. G. (2018) “The Ribosome: A Hot Spot for the Identification of New Types of Protein Methyltransferases” J Biol. Chem. 293, 10438-10446. PMCID: PMC6036201.
291. Roher, A. E., Kokjohn, T. A., Clarke, S. G., Sierks, M. R., Maarouf, C. L., Serrano, G. E., Sabbagh, M. S., and Beach, T. G. (2017) “APP/Aβ Structural Diversity and Alzheimer’s Disease Pathogenesis” Neurochemistry International, 110, 1-13. PMCID: PMC5688956.
290. Jain, K., Jin, C.Y., and Clarke, S.G. (2017) “Epigenetic control via allosteric regulation of mammalian protein arginine methyltransferases.” Proc. Natl. Acad. Sci. U. S. A. 114, 10101-10106. PMCID: 5617285.
289. Hadjikyriacou, A. and Clarke, S. G. (2017) “Caenorhabditis elegans PRMT-7 and PRMT-9 Are Evolutionarily Conserved Protein Arginine Methyltransferases with Distinct Substrate Specificities” Biochemistry. 56, 2612-2626. PMCID: 5503151.
288. Kafkova, L., Debler, E.W., Fisk, J.C., Jain, K., Clarke, S.G., and Read, L.K.(2016) “The Major Protein Arginine Methyltransferase in Trypanosoma brucei Functions as an Enzyme-Prozyme Complex” J. Biol. Chem. 292, 2089-2100. PMCID: 5313084.
287. Caslavka Zemple, K. E., Vashisht, A. A., Barshop, W. D., Wohlschlegel, J. A., and Clarke, S. G. (2016) “Determining the Mitochondrial Methylome in Saccharomyces cerevisiae using Heavy Methyl SILAC” J. Proteome Res. 15, 4436-4451. PMCID: 5148652.
286. Lowenson, J. D., Shmanai, V. V., Shklyaruck, D., Clarke, S. G., and Shchepinov, M. S. (2016) “Deuteration can protect an asparagine residues from racemization” Amino Acids 48, 2179-2188. PMCID: PMC4990494.
285. Jain, K., Warmack, R. A., Debler, E. W., Hadjikyriacou, A., Stavropoulos, P., and Clarke, S. G. (2016) “Protein arginine methyltransferase product specificity is mediated by distinct active-site architectures” J. Biol. Chem. 291, 18299-18308. PMCID: PMC5000077.
284. Warmack, R. A., Mansilla, E., Goya, R. G., and Clarke, S. G. (2016) “Racemized and Isomerized Proteins in Aging Rat Teeth and Eye Lens” Rejuvenation Res. 19, 309-317. PMCID: PMC4971426.
283. Sherkhanov, S., Korman, T. P., Clarke, S. G., and Bowie, J. U. (2016) “Production of FAME biodiesel in E. coli by direct methylation with an insect enzyme” Sci. Rep. 6:24239. DOI: 10.1038/srep24239. PMCID: PMC4823748.
282. Al-Hadid, Q., Roy, K., Chanfreau, G., and Clarke, S. G. (2016) “Methylation of Yeast Ribosomal Protein Rpl3 Promotes Translational Elongation Fidelity” RNA 22, 489-499. PMCID: PMC4793205.
281. Debler, E. W., Jain, K., Warmack, R. A., Feng, Y., Clarke, S. G., Blobel, G., and Stavropolousa, P. (2016) “A Glutamate/Aspartate Switch Controls Product Specificity in a Protein Arginine Methyltransferase” Proc. Natl. Acad. Sci. U. S. A. 113, 2068-2073. PMCID: PMC4776506.
280. Al-Hadid, Q., White, J., and Clarke, S. (2016) “Ribosomal Protein Methyltransferases in the Yeast Saccharomyces cerevisiae: Roles in Ribosome Biogenesis and Translation” Biochem. Biophys. Res. Commun. 470, 552-557. PMCID: PMC4750496.
279. Fu, X., Chin, R. M., Vergenes, L., Hwang H., Deng, G., Xing, Y., Pai, M. Y., Li, S., Ta, L., Fazlollahi, F., Chen, C., Prins, R. M., Teitell, M. A., Nathanson, D. A., Lai, A., Faull, K. F., Jiang, M., Clarke, S. G., Cloughesy, T. F., Graeber, T. G., Brass, D., Cristofk, H. R., Jung, M. E., Reue, K., and Huang, J. (2015) “2-Hydroxyglutarate Inhibits ATP Synthase and mTOR Signaling” Cell Metabolism, 22,508-515. PMCID: PMC4663076.
278. Hadjikyriacou, A., Yang, Y., Espejo, A., Bedford, M.T., and Clarke, S.G. (2015) “Unique Features of Human Protein Arginine Methyltransferase 9 (PRMT9) and its Substrate RNA Splicing Factor SF3B2” Journal of Biological Chemistry, E pub.
277. Yang, Y., Hadjikyriacou, A., Xia, Z., Gayatri, S., Kim, D., Zurita-Lopez, C., Kelly, R., Guo, A., Li, W., Clarke, S.G., and Bedford, M.T. (2015) “PRMT9 is a Type II methyltransferase that methylates the splicing factor SAP145″ Nature Communications, 6:6428.
276. Patananan, A.N., Budenholzer, L.M., Pedraza, M.E., Torres, E.R., Adler, L.N., and Clarke, S.G. (2015) “The invertebrate Caenorhabditis elegans biosynthesizes ascorbate” Archives of Biochemistry and Biophysics. DOI: 10.1016/j.abb.2015.02.002.
275. Patananan, A.N., Budenholzer, L.M., Eskin, A., Torres, E.R., and Clarke, S.G. (2015) “Ethanol-induced differential gene expression and acetyl-CoA metabolism in a longevity model of the nematode Caenorhabditis elegans” Experimental Gerontology, 61, 20-30.
274. Dzialo, M.C., Travaglini, K.J., Shen, S., Loo, J.A., and Clarke, S.G. (2014) “A new type of protein lysine methyltransferase trimethylates Lys-79 of elongation factor 1A” Biochemical and Biophysical Research Communications, 455, 382-389.
273. Dzialo, M.C., Travaglini, K.J., Shen, S., Roy, K., Chanfreau, G.F., Loo, J.A., and Clarke, S.G. (2014) “Translational Roles of Elongation Factor 2 Protein Lysine Methylation” Journal of Biological Chemistry, 289, 30511-30524.
272. Feng, Y., Hadjikyriacou, A., and Clarke, S.G. (2014) “Substrate specificity of human protein arginine methyltransferase 7 (PRMT7): the importance of acidic residues in the double E loop” Journal of Biological Chemistry, 289, 32604-32616.
271. Al-Hadid, Q., Roy, K., Munroe, W., Dzialo, M.C., Chanfreau, G.F., and Clarke, S.G. (2014) “Histidine Methylation of Yeast Ribosomal Protein Rpl3p is Required for Proper 60S Subunit Assembly” Molecular and Cellular Biology, 34, 2903-2916.
270. Patananan, A.N., Capri, J., Whitelegge, J.P., and Clarke, S.G. (2014) “Non-Repair Pathways for Minimizing Protein Isoaspartyl Damage in the Yeast Saccharomyces cerevisiae” Journal of Biological Chemistry, 289, 16936-16953.
269. Feng, Y., Maity, R., Whitelegge, J.P., Hadjikyriacou, A., Li, Z., Zurita-Lopez, C., Al-Hadid, Q., Clark, A.T., Bedford, M.T., Masson, J.Y., and Clarke, S.G. (2013) “Mammalian protein arginine methyltransferase 7 (PRMT7) specifically targets RXR sites in lysine- and arginine-rich regions” Journal of Biological Chemistry, 288, 37010-25.
268. Lissina, E., Weiss, D., Young, B., Rella, A., Cheung-Ong, K., Del Poeta, M., Clarke, S. G., Giaever, G., and Nislow, C. (2013) “A Novel Small Molecule Methyltransferase is Important for Virulence in Candida albicans” ACS Chem. Biol. 8, 2785-2793. PMCID: PMC3928151.
267. Shen-Miller, J., Linder, P., Xie, Y., Villa, S., Wooding, K., Clarke, S.G., Loo, R.R.O, and Loo, J.A. (2013) “Thermal-Stable Proteins of Fruit of Long-Lived Sacred Lotus Nelumbo nucifera Gaertn var China Antique” Tropical Plant Biology, 6, 69-84.
266. Yang, H., Lowenson, J.D., Clarke, S., and Zubarev, R.A. (2013) “Brain Proteomics Supports the Role of Glutamate Metabolism and Suggests Other Metabolic Alterations in Protein L-Isoaspartyl Methyltransferase (PIMT)-Knockout Mice” J. Proteome Research, 12, 4566-4576.
265. Nayak, N.R., Putnam, A.A., Addepalli, B., Lowenson, J.D., Chen, T., Jankowsky, E., Perry, S.E., Dinkins, R.D., Limbach, P.A., Clarke, S.G., and Downie, A.B. (2013) “An Arabidopsis ATP-Dependent, DEAD-Box RNA Helicase Loses Activity Upon IsoAsp Formation but Is Restored by Protein Isoaspartyl Methyltransferase” Plant Cell, 25, 2573-2586.
264. MacKay, K. B., Tu., Y., Young, S. G., and Clarke, S. G. (2013) “Circumventing Embryonic Lethality with Lcmt1 Deficiency: Generation of Hypomorphic Lcmt1 Mice with Reduced Protein Phosphatase 2A Methyltransferase Expression and Defects in Insulin Signaling” PLos One, 8, 1-11.
263. Patananan, A. N., Palmer, J. M., Garvey, G. S., Keller, N. P., and Clarke, S. G. (2013) “A Novel Automethylation Reaction in the Aspergillus nidulans LaeA Protein Generates S-Methylmethionine” J. Biol. Chem. 288, 20, 14032-14045.
262. Clarke, S. G. (2013) “Protein Methylation at the Surface and Buried Deep: Thinking Outside the Histone Box” Trends in Biochemical Sciences, May 2013, 38, 5, 243-252.
261. Dai, S., Ni, W., Patananan, A. N., Clarke, S. G., Karger, B. L., and Zhou, Z. S. (2013) “Integrated Proteomic Analysis of Major Isoaspartyl-Containing Proteins in the Urine of Wild Type and Protein L-Isoaspartate O-Methyltransferase-Deficient Mice” Analytical Chemistry 2013, 85, 2423-2430.
260. Dhar, S., Vemulapalli, V., Patananan, A. N., Huang, G. L., Di Lorenzo, A., Richard, S., Comb, M. J., Guo, A., Clarke, S. G., and Bedford, M. T. (2013) “Loss of the major Type I arginine methyltransferase PRMT1 causes substrate scavenging by other PRMTs” Scientific Reports, 3:3011, 1-6.
259. Yang, M-L., Gee, A. J. P., Gee, R. J., Zurita-Lopez, C. I., Khare, S., Clarke, S. G., and Mamula, M. J. (2013) “Lupus Autoimmunity Altered by Cellular Methylation Metabolism” Autoimmunity, 46(1), 21-31.
258. Patananan, A. N., and Clarke, S. G. (2013) “Isoaspartyl Dipeptidase (IadA)” In Handbook of Proteolytic Enzymes, Vol. 1, Ed. Rawlings, N. D., and Salvesen, G. S., Oxford: Academic Press, 1654-1663.
257. Urzica, E. I., Casero, D., Yamasaki, H., Hsieh, S., Adler, L. N., Karpowicz, S. J., Blaby-Haas, C. E., Clarke, S. G., Loo, J. A., Pellegrini, M., and Merchant, S. M. (2012) “Systems and Trans-System Level Analysis Identifies Conserved Iron Defciency Responses in the Plant Lineage” The Plant Cell 24, 3921-3948.
256. MacKay, K., Lowenson, J. D., and Clarke, S. G. (2012) “Wortmannin Reduces Insulin Signaling and Death in Seizure-Prone Pcmt1-/- Mice” PLOS One, 7, 1-14.
255. Young, B. D., Weiss, D. I., Zurita-Lopez, C. I., Webb, K. J., Clarke, S., G., and McBride, A. E. (2012) “Identification of Methylated Proteins in the Yeast Small Ribosomal Subunit: A Role for SPOUT Methyltransferases in Protein Arginine Methylation” Biochemistry 51, 5091-5104.
254. Urzica, E. I., Adler, L. N., Page, M. D., Linster, C., Arbing, M. A., Casero, D., Pellegrini, M., Merchant, S. S., and Clarke, S. G. (2012) “Impact of Oxidative Stress on Ascorbate Biosynthesis in Chlamydomonas via Regulation of the VTC2 Gene Encoding a GDP-L-galactose Phosphorylase” J. Biol. Chem. 287, 17, 14234-14245.
253. Zurita-Lopez, C. I., Sandberg, T., Kelly, R., and Clarke, S.G. (2012) “Human Protein Arginine Methyltransferase 7 (PRMT7) Is a Type III Enzyme Forming ω -NG-Monomethylated Arginine Residues” J. Biol. Chem. 287, 11, 7859-7870.
252. Castro, P. V., Khare, S., Young, B. D., and Clarke, S. G. (2012) “Caenorhabditis elegans Battling Starvation Stress: Low Levels of Ethanol Prolong Lifespan in L1 Larvae” PLos One, 7, 1-12.
Steven Clarke Lab Publications – 2008 – 2011
251. Lissina, E., Young, B., Urbanus, M. L., Guan, X. L., Lowenson, J., Hoon, S., Baryshnikova, A., Riezman, I., Michaut, M., Riezman, H., Cowen, Leah E., Wenk, M., Clarke, S. G., Giaever, G., and Nislow, C. (2011) “A Systems Biology Approach Reveals the Role of a Novel Methyltransferase in Response to Chemical Stress and Lipid Homeostasis” PLoS Genetics, 7, 1-16.
250. Adler, L. N., Gomez, T. A., Clarke, S. G., and Linster, C. L. (2011) “A Novel GDP-D-glucose Phosphorylase Involved in Quality Control of the Nucleoside Diphosphate Sugar Pool in Caenorhabditis elegans and Mammals” J. Biol. Chem. 286, 24, 21511–21523.
249. Khare, S., Linster, C. L., and Clarke, S. G. (2011) “The Interplay between Protein L-Isoaspartyl Methyltransferase Activity and Insulin-Like Signaling to Extend Lifespan in Caenorhabditis elegans” PLos One, 6, 1-12.
248. Webb., K. J., Al-Hadid, Q., Zurita-Lopez, C. I., Young, B. D., Lipson, R. S., and Clarke, S. G. (2011) “The Ribosomal L1 Protuberance in Yeast Is Methylated on a Lysine Residue Catalyzed by a Seven-β-strand Methyltransferase” J. Biol. Chem. 286, 21, 18405–18413.
247. Rust, H. L., Zurita-Lopez. C. I., Clarke, S. G., Thompson, P.R. (2011) “Mechanistic Studies on Transcriptional Coactivator Protein Arginine Methyltransferase 1” Biochemistry 50, 3332–3345.
246. Butler, J. S., Zurita-Lopez, C. I., Clarke, S. G., Bedford, M. T., Dent, S. Y. R. (2011) “Protein-arginine Methyltransferase 1 (PRMT1) Methylates Ash2L, a Shared Component of Mammalian Histone H3K4 Methyltransferase Complexes” J. Biol. Chem. 286, 14, 12234–12244.
245. Petrossian, T. C., and Clarke, S. G. (2011) “Uncovering the Human Methyltransferasome” Molecular & Cellular Proteomics 10.1, 1-12.
244. Webb, K. J., Zurita-Lopez, C. I., Al-Hadid, Q., Laganowsky, A., Young, B. D., Lipson, R. S., Souda, P., Faull, K. F., Whitelegge, J. P., and Clarke, S. G. (2010) “A Novel 3-Methylhistidine Modification of Yeast Ribosomal Protein Rpl3 Is Dependent upon the YIL110W Methyltransferase” J. Biol. Chem. 285, 48, 37598–37606.
243. Chen, T., Nayak, N., Majee, S. M., Lowenson, J., Schafermeyer, K. R., Eliopoulos, A. C., Lloyd, T. C., Dinkins, R., Perry, S., Forsthoefel, N. R., Clarke, S. G., Vernon, D. M., Zhou, Z. S., Rejtar, T., and Downie, A. B. (2010) “Substrates of the Arabidopsis thaliana Protein Isoaspartyl Methyltransferase 1 Identified Using Phage Display and Biopanning” J. Biol. Chem. 285, 48, 37281–37292.
242. Lipson, R. S., Webb, K. J., and Clarke, S. G. (2010) “Two novel methyltransferases acting upon eukaryotic elongation factor 1A in Saccharomyces cerevisiae” Archives of Biochemistry and Biophysics 500, 137-143.
241. Vinci, C. R., and Clarke, S. G. (2010) “Homocysteine Methyltransferases Mht1 and Sam4 Prevent the Accumulation of Age-damaged (R,S)-AdoMet in the Yeast Saccharomyces cerevisiae” J. Biol. Chem. 285, 27, 20526–20531.
240. Webb, K. J., Lipson, R. S., Al-Hadid, Q., Whitelegge, J. P., and Clarke, S. G. (2010) “Identification of Protein N-Terminal Methyltransferases in Yeast and Humans” Biochemistry, 49, 25, 5225–5235.
239. Fisk, J. C., Zurita-Lopez, C., Sayegh, J., Tomasello, D. T., Clarke, S. G., and Read, L. K. (2010) “TbPRMT6 Is a Type I Protein Arginine Methyltransferase That Contributes to Cytokinesis in Trypanosoma brucei” Eukaryotic Cell, Volume 9., No. 6, 866-877.
238. Vinci, C.R. and Clarke, S.G. (2010) “Yeast, Plants, Worms, and Flies Use a Methyltransferase to Metabolize Age-Damaged (R,S)-AdoMet, but What Do Mammals Do?” Rejuvenation Research,
Volume 13, Number 2-3, 362-364.
237. Lipson, R. S., Webb, K. J., and Clarke, S. G. (2010) “Rmt1 catalyzes zinc-finger independent arginine methylation of ribosomal protein Rps2 in Saccharomyces cerevisiae” Biochemical and Biophysical Research Communications 391, 1658–1662.
236. Lakowski, T.M, Zurita-Lopez, C., Clarke, S.G., and Frankel, A. (2010) “Approaches to measuring the activities of protein arginine N-methyltransferases” Analytical Biochemistry 397, 1-11.
235. Petrossian, T. C., and Clarke, S. G. (2009) “Bioinformatic Identification of Novel Methyltransferases” Epigenomics 1, 163-175.
Poster Presentation: Petrossian, T. C. and Clarke, S. G. (2009) “Computational methods to identify novel methyltransferases” BMC Bioinformatics 10 (Suppl. 13) 7-8.
234. Khare, S., Gomez, T. A., Linster, C. L. and Clarke, S. G. (2009) “Defective Responses to Oxidative Stress in Protein L-Isoaspartyl Repair-Deficient Caenorhabditis elegans” Mechan. Ageing Develop. 130, 670-680.
233. Petrossian, T. C., and Clarke, S. G. (2009) “Multiple Motif Scanning to Identify Methyltransferases from the Yeast Proteome” Mol. Cell. Proteomics 8, 1516-1526.
232. Fisk, J. C., Sayegh, J., Zurita-Lopez, C., Menon, S., Presnyak, V., Clarke, S. G., and Read, L. K. (2009) “A Type III Protein Arginine Methyltransferase from the Protozoan Parasite, Trypanosoma brucei” J. Biol. Chem. 284, 11590-11600.
231. Bedford, M. T., and Clarke, S. G. (2009) “Protein Arginine Methylation in Mammals: Who, What and Why” Mol. Cell 33, 1-13.
230. Webb, K. J., Laganowsky, A., Whitelegge, J. P., and Clarke, S. G. (2008) “Identification of Two SET-Domain Proteins Required for Methylation of Lysine Residues in Yeast Ribosomal Protein Rpl42ab” J. Biol. Chem. 283, 35561-35568.
229. Gomez, T. A., Banfield, K. L., and Clarke, S. G. (2008) “The Protein L-Isoaspartyl-O-Methyltransferase Functions in the Caenorhabditis elegans Stress Response” Mechan. Ageing Develop. 129, 752-758.
228. Linster, C. L., and Clarke, S. G. (2008) “L-Ascorbate Biosynthesis in Higher Plants: Role of VTC2” Trends Plant Sci. 13, 567-573. (Article featured as a cover illustration). PMCID:
227. Banfield, K. L., Gomez, T. A., Lee, W., Clarke, S., and Larsen, P. L.(2008) “Protein-Repair and Hormone-Signaling Pathways Specify Dauer and Adult Longevity and Dauer Development in Caenorhabditis elegans” J. Gerontol. A. Biol. Sci. Med. Sci. 63A, 798-808.
226. Sayegh, J., and Clarke, S. (2008) “Hsl7 is a Substrate-specific Type II Protein Arginine Methyltransferase in Yeast” Biochem. Biophys. Res. Commun. 372, 811-815.
225. Linster, C. L., Adler, L. N., Webb, K., Christensen, K. C., Brenner, C., and Clarke, S. G. (2008) “A Second GDP-l-Galactose Phosphorylase in Arabidopsis en Route to Vitamin C: Covalent Intermediate and Substrate Requirements for the Conserved Reaction” J. Biol. Chem. 283, 18483-18492.
224. Alfaro, J. F., Gillies, L. A., Sun, H. G., Dai, S., Zang, T., Klaene, J. J., Kim, B. J., Lowenson, J. D., Clarke, S. G., Karger, B. L., and Zhou, Z. S. (2008) “Chemo-Enzymatic Detection of Protein Isoaspartate Using Protein Isoaspartate Methyltransferase and Hydrazine Trapping” Anal. Chem. 80, 3882-3889.
223. Dumlao, D. S., Hertz, N., and Clarke, S. (2008) “Secreted “3-Isopropylmalate Methyl Ester Signals Invasive Growth During Amino Acid Starvation in Saccharomyces cerevisiae” Biochemistry, 47 (2), 698 -709, 2008.
Steven Clarke Lab Publications – 2004 – 2007
222. Sayegh, J., Webb, K., Cheng, D., Bedford, M. T., and Clarke, S. G. (2007) “Regulation of Protein Arginine Methyltransferase 8 (PRMT8) Activity by its N Terminal Domain” J. Biol. Chem. 282, 36444-36453.
221. Lipson, R. S., and Clarke, S. G. (2007) “S-Adenosylmethionine-dependent protein methylation in mammalian cytosol via tyrphostin methylation by catechol-O-methyltransferase” J. Biol. Chem. 282, 31094-31102.
220. Pasternack, D. A., Sayegh, J., Clarke, S., and Read, L. K. (2007) “Evolutionarily Divergent Type II Protein Arginine Methyltransferase in Trypanosoma bruceii” Eukaryotic Cell 6, 1665-1681.
219. Clarke, S. G. (2007) “HIV protease inhibitors and nuclear lamin processing: getting the right bells and whistles” Proc. Natl. Acad. Sci. U. S. A. 104, 13857-13858.
218. Gomez, T. A., and Clarke, S. G. (2007) “Autophagy and Insulin/TOR Signaling in Caenorhabditis elegans pcm-1 protein repair mutants” Autophagy 3, 357- 359.
217. Chen, H., Xia, M., Lin, M., Yang, H., Kuhlenkamp, J., Li, T., Sodir, N. M., Josef-Lenz, H., Laird, P. W., Clarke, S., Mato, J., M., and Lu, S. C. (2007) “Role of Methionine Adenosyltransferase 2A and S-Adenosylmethionine in Mitogen-Induced Growth of Human Colon Cancer Cells” Gastroenterology 133, 207-218.
216. McBride, A. E., Zurita-Lopez, C., Regis, A., Blum, E., Conboy, A., Elf, S., and Clarke, S. (2007) “Protein Arginine Methylation in Candida albicans: Role in Nuclear Transport” Eukaryotic Cell 6, 1119-1129.
215. Zou, Y., Webb, K., Perna, A., Clarke, S., and Wang, Y. (2007) “A Mass Spectrometric Study on the In-vitro Methylation of HMGA1a and HMGA1b proteins by PRMTs: Methylation Specificity, the Effect of Binding to AT- rich Duplex DNA, and the Effect of C-terminal Phosphorylation” Biochemistry 46, 7896-7906.
214. Linster, C. L., Gomez, T. A., Christensen, K. C., Adler, L., Young, B. D., Brenner, C. and Clarke, S. G. (2007) “Arabidopsis VTC2 encodes a GDP-L-galactose phosphorylase, the last unknown enzyme in the Smirnoff-Wheeler pathway to ascorbic acid in plants.” J. Biol. Chem. 282, 18879-18885. Journal of Biological Chemistry “Paper of the Week”.
213. Porras-Yakushi, T. R., Whitelegge, J. P., and Clarke, S. (2007) “Yeast Ribosomal Cytochrome c SET-domain Methyltransferase Subfamily: Identification of Rpl23ab Methylation Sites and Recognition Motifs” J. Biol Chem. 282, 12368-12376.
212. Vinci, C. R., and Clarke, S. G. (2007) “Recognition of Age-Damaged (R,S)AdoMet by Two Methyltransferases in the Yeast Saccharomyces Cerevisiae” J. Biol Chem. 282, 8604-8612.
211. Gomez, T. A., Banfield, K. L., Trogler, D. M., and Clarke, S. (2007) “The L- Isoaspartyl-O-Methyltransferase in Caenorhabditis elegans Larval Longevity and Autophagy” Develop. Biol. 303, 493-500.
210. Porras-Yakushi, T. R., Whitelegge, J. P., and Clarke, S. (2006) “A Novel SET-Domain Methyltransferase in Yeast: Rkm2-dependent Trimethylation of Ribosomal Protein L12ab at Lysine 10” J. Biol Chem. 281, 35835-35845.
209. Villa, S. T., Xu, Q., Downie, A. B., and Clarke, S. (2006) “Arabidopsis Protein Repair L-Isoaspartyl Methyltransferases: Predominant Activities at Lethal Temperatures” Physiol. Planta. 128, 581-592.
208. Vigneswara, V., Lowenson, J. D., Powell, C. D., Thakur, M., Clarke, S., Ray, D. E., and Carter, W. G. (2006) “Proteomic Identification of Novel Substrates of a Protein Isoaspartyl Methyltransferase Repair Enzyme” J. Biol. Chem. 281, 32619-32629.
207. Yang, M.-L., Doyle, H. A., Gee, R. J., Lowenson, J. D., Clarke, S., Lawson, B. R., Aswad, D. W., and Mamula, M. J. (2006) “Intracellular Protein Modification Associated with Altered T Cell Functions in Autoimmunity” J. Immunol. 177, 4541-4549.
206. Clarke, S. G. (2006) “Inhibition of Mammalian Protein Methyltransferases by 5′-Methylthioadenosine (MTA): A Mechanism of Action of Dietary SAMe?” in Protein Methyltransferases, a volume of The Enzymes, 3rd edition (Clarke, S. G. and Tanamoni, F., eds), 24, 467-493.
205. Young, S. G., Clarke, S. G., Bergo, M. O., Philips, M., and Fong, L. G. (2006) “Genetic Approaches for Understanding the Physiological Importance of the Carboxyl Methylation of Isoprenylated Proteins” in Protein Methyltransferases, a volume of The Enzymes, 3rd edition (Clarke, S. G. and Tanamoni, F., eds), 24, 273-301.
204. Miranda, T. B., Sayegh, J., Frankel, A., Katz, J. E., Miranda, M., and Clarke, S. (2006) “Yeast Hsl7 (histone synthetic lethal 7) Catalyzes the in vitro Formation of ?-NG-Monomethylarginine in Calf Thymus Histone H2A” Biochem. J. 395, 563-570.
203. Stetler, A., Winograd, C., Sayegh, J., Cheever, A., Patton, E., Zhang, X., Clarke, S., and Ceman, S. (2006) “Identification and characterization of the methyl arginines in the fragile X mental retardation protein Fmrp” Human Mol. Gen. 15, 87-96.
202. Farrar, C. E., Huang, C. S., Clarke, S. G., and Houser, C. R. (2005) “Increased Cell Proliferation and Granule Cell Number in the Dentate Gyrus of Protein Repair-Deficient Mice” J. Comparative Neurol. 493, 524-537.
201. Miranda, T. B., Webb, K. J., Edberg, D. D., Reeves, R., and Clarke, S. (2005) “Protein Arginine Methyltransferase 6 (PRMT6) Specifically Methylates the Nonhistone Chromosomal Protein HMGA1a” Biochem. Biophys. Res. Commun.
336, 831-835.
200. Porras-Yakushi, T. R., Whitelegge, J. P., Miranda, T. B., and Clarke, S. (2005) “A Novel SET-Domain Methyltransferase Modifies Ribosomal Protein Rpl23ab in Yeast” J. Biol. Chem. 280 34590-34598.
199. Lee, J., Sayegh, J., Daniel, J., Clarke, S., and Bedford, M. T. (2005) “PRMT8: A New Membrane-Bound Tissue-Specific Member of the Protein Arginine Methyltransferase Family” J. Biol. Chem. 280, 32890-32896.
198. Farrar, C. E., and Clarke, S. G. (2005) “Diet-Dependent Survival of Protein Repair-Deficient Mice” J. Nutr. Biochem. 16, 554-561.
197. Kalhor, H. R., Penjwini, M., and Clarke, S. (2005) “A Novel methyltransferase required for the formation of the hypermodified nucleoside wybutosine in eucaryotic tRNA” Biochem. Biophys. Res. Commun. 334, 443-440.
196. Christine E. Farrar, Carolyn R. Houser, and Steven Clarke (2005) “Activation of the PI3K/Akt Signal Transduction Pathway and Increased Levels of Insulin Receptor in Protein Repair�Deficient Mice” Aging Cell 4, 1-12.
195. Tina B. Miranda, J. D. Lowenson, and Steven Clarke (2004) “A New Type of Protein Methylation Activated by TyrphostinA25 and Vanadate” FEBS Lett. 577, 181-186.
194. Tina Branscombe Miranda, Permanan Khusial, Jeffry R. Cook, Jin-hyung Lee, Samuel I. Gunderson, Sidney Pestka, Gary W. Zieve, and Steven Clarke. (2004) “Spliceosome Sm proteins D1, D3, and B/B’ are asymmetrically dimethylated at arginine residues in the nucleus” Biochem. Biophys. Res. Commun. 323, 181-186.
193. Vinita Singh, Tina Branscombe Miranda, Wei Jiang, Adam Frankel, Martha E. Roemer, Victoria A. Robb, David H. Gutmann, Harvey R. Herschman, H. R., Steven Clarke, and Irene F. Newsham (2004) “DAL‑1/4.1B Tumor Suppressor Interacts with Protein Arginine N-Methyltransferase 3 (PRMT3) and Inhibits its Ability to Methylate Substrates in vitro and in vivo” Oncogene 23, 7761-7771.
192. Yanming Wang, Joanna Wysocka, Joyce Sayegh, Young-Ho Lee, Julie R. Perlin, Lauriebeth Leonelli, Lakshmi S. Sonbuchner, Charles H. McDonald, Richard G. Cook, Yali Dou, Robert G. Roeder, Steven Clarke, Michael R. Stallcup, C. David Allis, and Scott A. Coonrod (2004) “Human PAD4 Regulates Histone Arginine Methylation Levels via Demethylimination” Science 306, 279-283.
191. Qilong Xu, Marisa P. Belcastro, Sarah T. Villa, Randy D. Dinkins, Steven G. Clarke, and A. Bruce Downie (2004) “A Second Protein L-Isoaspartyl Methyltransferase Gene in Arabidopis Produces Two Transcripts Whose Products are Sequestered in the Nucleus” Plant Physiology 136, 2552-2664.
190. Vitali, R., and Clarke, S. (2004) “Improved rotorod performance and hyperactivity in mice deficient in a protein repair methyltransferase” Behavioral Brain Res. 153, 129-141.
189. Gary, J. D., and Clarke, S. (2004) “IadA b-Aspartyl Dipeptidase” in Handbook of Proteolytic Enzymes, 2nd edition, (eds. Woessner, G., Rawlings, N., and Barrett, A. J.), Academic Press, New York.
188. Miranda, T. B., Miranda, M., Frankel, A., and Clarke, S. (2004) “PRMT7 is a Member of the Protein Arginine Methyltransferase Family with a Distinct Substrate Specificity” J. Biol. Chem. 279, 22902-22907.
187. Katz, J. E., Dumlao, D. S., Wasserman, J. I., Lansdown, M. G., Jung, M. E., Faull, K. F., and Clarke, S. (2004) “3-Isopropylmalate is the Major Endogenous Substrate of the Saccharomyces cerevisiae trans-Aconitate Methyltransferase” Biochemistry 43, 5976-5986.
186. Boulanger, M.-C., Miranda, T. B., Clarke, S., Di Fruscio, M., Suter, B., Lasko, P., and Richard, S. (2004) “Characterization of Drosophila Protein Arginine Methyltransferases DART1 and DART4” Biochem. J. 379, 283-289.
185. Katz, J. E., Hau, J., Dumlao, D. S., and Clarke, S. (2004) “A New Technique (COMSPARI) to Facilitate the Identification of Minor Compounds in Complex Mixtures by GC/MS and LC/MS: Tools for the Visualization of Matched Datasets” J. Amer. Soc. Mass Spectrom. 15, 580-584.
184. Clarke, S., and Tamanoi, F. (2004) “Fighting Cancer by Disrupting C-Terminal Methylation of Signaling Proteins” J. Clin. Invest. 113. 513-515.
Steven Clarke Lab Publications – 2000 – 2003
183. Kalhor, H. R., and Clarke, S. (2003) “Novel Methyltransferase for Modified Uridine Residues at the Wobble Position of tRNA” Mol. Cell Biol. 23, 9283- 9292.
182. Katz, J. E., Dlakic, M., and Clarke, S. (2003) “Automated Identification of Putative Methyltransferases from Genomic Open Reading Frames” Mol. Cell. Proteomics 2, 525-540.
181. Clarke, S. (2003) “Aging as War Between Chemical and Biochemical Processes: Protein Methylation and the Recognition of Age-Damaged Proteins for Repair” Ageing Res. Rev. 2, 263-285.
180. Mura, C., Katz, J. E., Clarke, S. G., and Eisenberg, D. (2003) “Structure and Function of an Archaeal Homolog of Survival Protein E (SurE): An Acid Phosphatase with Purine Nucleotide Specificity” J. Mol. Biol. 326, 1559-1575.
179. Farrar, C., and Clarke, S. (2002) “Altered Levels of S-Adenosylmethionine and S-Adenosylhomocysteine in the Brains of L-Isoaspartyl (D-Aspartyl) O-Methyltransferase-deficient Mice” J. Biol. Chem. 277, 27856-27863.
178. Clarke, S., and Banfield, K. (2002) “Can Elevated Plasma Homocysteine Levels Result in the Inhibition of Intracellular Methyltransferases” in Chemistry and Biology of Pteridines and Folates (Milstein, S., Kapatos, G., Levine, R. A., and Shane, B., eds), pp. 557-562, Kluwer Academic Publishers, Boston.
177. Ryttersgaard, C., Griffith, S. C., Sawaya, M. R., MacLaren, D. C., Clarke, S., and Yeates, T. O. (2002) “Crystal structure of human L-isoaspartyl methyltransferase” J. Biol. Chem. 277, 10642-10646.
176. Clarke, S. (2002) “Commentary: The Methylator Meets the Terminator” Proc. Natl. Acad. Sci. U. S. A. 99, 1104-1106.
175. Frankel, A., Yadav, N., Lee, J., Branscombe, T. L., Clarke, S., and Bedford, M. T. (2002) “The Novel Human Protein Arginine N-Methyltransferase PRMT6 is a Nuclear Enzyme Displaying Unique Substrate Specificity” J. Biol. Chem. 277, 3537-3543.
174. Thapar, N., Griffith, S., Yeates, T. O., and Clarke, S. (2002).“Protein Repair Methyltransferase from the Hyperthermophilic Archaeon Pyrococcus furiosus: Unusual Methyl-accepting Affinity for D-Aspartyl and N-Succinyl- containing Peptides” J. Biol. Chem. 277, 1058-1065.
173. Kalhor, H. R., Luk, K., Ramos, A., Zobel-Thropp, P., and Clarke, S. (2001) “Protein Phosphatase Methyltransferase 1 (PPM1) is the Sole Activity Responsible for Modification of the Major forms of Protein Phosphatase 2A in Yeast” Arch. Biochem. Biophys. 395, 239-245.
172. Cai, H., Dumlao, D., Katz, J. E. , and Clarke, S. (2001) “Identification of the Gene and Characterization of the Activity of the trans-Aconitate Methyltransferase from Saccharomyces cerevisiae” Biochemistry 40, 13699- 13709.
171. Griffith, S. C., Sawaya, M. R., Boutz, D. R., Thapar, N., Katz, J. E., Clarke, S., and Yeates, T. O. (2001) “Crystal Structure of a Protein Repair Methyltransferase from Pyrococcus furiosus with its L-Isoaspartyl Peptide Substrate” J. Mol. Biol. 313, 1103-1116.
170. Zhang, R.-G., Skarina, T., Katz, J. E., Beasley, S., Khachatryan, A., Vyas, S., Arrowsmith, C. H., Clarke, S., Edwards, A., Joachimiak, A., and Savchenko, A. (2001) “Structure of Thermotoga maritima SurE – Stationary Phase Survival Protein – a Novel Acid Phosphatase” Structure 9, 1095-1096.
169. Branscombe, T. L., Frankel, A., Lee, J.-H., Cook, J. R., Yang, Z., Pestka, S., and Clarke, S. (2001) “PRMT5 (the Janus Kinase Binding Protein 1) Catalyzes the Formation of Symmetric Dimethylarginine Residues in Proteins” J. Biol. Chem. 276, 32971-32976.
168. Clarke, S., and Banfield, K. (2001) “S-Adenosylmethionine-dependent Methyltransferases” in Homocysteine in Health and Disease (Carmel, R., and Jacobsen, D., eds), pp. 63-78.
167. Lowenson, J. D., Kim, E., Young, S. G., and Clarke, S. (2001) “Limited Accumulation of Damaged Proteins in L-Isoaspartyl (D-Aspartyl) O- Methyltransferase-deficient Mice” J. Biol. Chem. 276, 20695-20702.
166. Radkiewicz, J. L., Zipse, H., Clarke, S., and Houk, K. N. (2001) “Neighboring Side Effects on Asparaginyl and Aspartyl Degradation: An Ab Initio Study of the Relationship Between Peptide Conformation and Backbone NH Acidity” J. Amer. Chem. Soc. 123, 3499-3506.
165. Cai, H., Strouse, J., Dumlao, D., Jung, M. E., and Clarke, S. (2001) “Distinct Reactions Catalyzed by Bacterial and Yeast trans-Aconitate Methyltransferases” Biochemistry 40, 2210-2219.
164. Thapar, N., Kim, A.-K., and Clarke, S. (2001) “Distinct Patterns of Expression but Similar Biochemical Properties of Protein L-Isoaspartyl Methyltransferases in Plants” Plant Physiol. 125, 1023-1035.
163. Zobel-Thropp, P., Yang, M. C., Machado, L., and Clarke, S. (2000) “A Novel Post-translational Modification of Yeast Elongation Factor 1A: Methylesterification at the C-Terminus” J. Biol. Chem. 275, 37150-37158.
162. Thapar, N., and Clarke, S. (2000) “Expression, Purification, and Characterization of the Protein Repair L-Isoaspartyl Methyltransferase from Arabidopsis thaliana” Protein Expression Purification 20, 237-251.
161. Frankel, A., and Clarke, S. (2000) “PRMT3 is a Distinct Member of the Protein Arginine N-Methyltransferase Family: Conferral of Substrate Specificity by a Zinc-Finger Domain” J. Biol. Chem. 275, 32974-32982.
160. Yan, S. D., Zhu, Y., Zhu, H., Stern, E. D., Hwang, Y. C., Hori, O., Ogawa, S., Frosch, M. P., Connolly, E. S., Jr., McTaggert, R., Pinsky, D. J., Clarke, S., Stern, D. M., and Ramasamy, R. (2000) “Amyloid Beta-Peptide Binding Alcohol Dehydrogenase is a Component of the Cellular Response to Nutritional Stress” J. Biol. Chem. 275, 27100-27109.
159. Young, S. G., Ambroziak, P., Kim, E., and Clarke, S. (2000) “Post- isoprenylation Protein Processing: CXXX (CaaX) Endoproteases and Isoprenylcysteine Carboxyl Methyltransferase” in Protein Lipidation, a volume of The Enzymes, 3rd edition (Tamanoi, F., and Sigman, D. S., eds), 21, 155-213.
158. Bedford, M. T., Frankel, A., Yaffe, M. B., Clarke, S., Leder, P. and Richard, S. (2000) “Arginine Methylation Inhibits the Binding of Proline- rich Ligands to Src Homology 3, but not WW Domains” J. Biol. Chem. 275, 16030-16036.
157. Tang, J., Frankel, A., Cook, R. J., Kim S., Paik, W. K., Williams, K. R., Clarke, S., and Herschman, H. R. (2000) “PRMT1 is the Predominant Type I Protein Arginine Methyltransferase in Mammalian Cells” J. Biol. Chem. 275, 7723-7730.